Supercritical Fluid Chromatography (SFC) is an advanced analytical technique that separates chemical compounds using a supercritical fluid—commonly carbon dioxide—as the mobile phase. Combining the properties of gases and liquids, supercritical fluids allow fast, efficient separations with reduced solvent usage. SFC is widely used in pharmaceuticals, natural products, petrochemicals, and chiral compound analysis for high-resolution, environmentally friendly separations. The technique is especially valuable for thermally sensitive or non-volatile compounds and offers high throughput with minimal sample degradation.